LOCALIZATION OF CHLORTETRACYCLINE AND VITAMIN B12 IN SUBGELLULAR FRACTIONS OF STREPTOMYCES AUREOFACIENS
نویسندگان
چکیده
منابع مشابه
Effect of temperature on the coproduction of chlortetracycline and tetracycline by Streptomyces aureofaciens.
Five strains of Streptomyces aureofaciens of diverse origin and ability to incorporate chloride into chlortetracycline (CTC) were grown at five temperatures of incubation ranging from 20 to 31 C in media of high and low chloride content. Maximal accumulation of CTC and tetracycline (TC) occurred in the 23 to 25 C range. Departure from this range affected the different strains to various degrees...
متن کاملMetabolic Behavior and Chlortetracycline Production by Streptomyces aureofaciens in Liquid Culture.
Standard fermentations were performed as follows: A spore suspension was inoculated into 3 L of the following medium: sucrose, 3 per cent; corn steep liquor, 1 per cent; molasses, 0.1 per cent; NaCl, 0.05 per cent; and CaCO3, 0.4 per cent. The fermentations were carried out at 27 C in 5-L fermentors (Bartholomew, Karow and Sfat, 1950). After 36 hours, 100 ml of the culture fluid was transferred...
متن کاملIdentification and cloning of the gene involved in the final step of chlortetracycline biosynthesis in Streptomyces aureofaciens.
For chlortetracycline biosynthesis in Streptomyces aureofaciens, the final reduction step is essential to give an antibiotic activity to its intermediate, which is catalyzed by tetracycline dehydrogenase with 7,8-dedimethyl-8-hydroxy-5-deazariboflavin (FO) as a cofactor. We identified and cloned the gene, which is essential for the biosynthesis of 6-demethyltetracycline and participates in the ...
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abstract هنجارهای بومی سازی در ترجمه متون چندوجهی فارسی:مورد دموهای بازیهای کامپیوتری چکیده اهداف عمده مطالعه حاضر به سه دسته تقسیم میشوند: 1) بررسی مشکلات احتمالی ترجمه دموهای (فیلمهای) بازیهای کامپیوتری،2) تعیین هنجارهای بومی سازی در ترجمه دموهای (فیلمهای) بازیهای کامپیوتری و 3) تعیین ایدئولوژیهایی که این هنجارها در جامعه نشان میدهند. به این منظور، ابتدا، مجموعه ای ازدموهای (فیلمهای) ب...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1968
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.21.363